Device

Part:BBa_K1998000:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-12)


Mg-Chelatase Plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4753
    Illegal NotI site found at 2666
    Illegal NotI site found at 4213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 439
    Illegal BglII site found at 1095
    Illegal BglII site found at 1290
    Illegal BglII site found at 3371
    Illegal BglII site found at 3898
    Illegal BglII site found at 5755
    Illegal BglII site found at 5863
    Illegal BamHI site found at 487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1946
    Illegal NgoMIV site found at 2765
    Illegal NgoMIV site found at 3307
    Illegal NgoMIV site found at 7968
    Illegal AgeI site found at 6853
    Illegal AgeI site found at 6883
    Illegal AgeI site found at 7777
    Illegal AgeI site found at 7945
    Illegal AgeI site found at 9463
    Illegal AgeI site found at 9517
    Illegal AgeI site found at 9736
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5553
    Illegal BsaI.rc site found at 5709
    Illegal BsaI.rc site found at 6330
    Illegal BsaI.rc site found at 9862
    Illegal SapI.rc site found at 7219
    Illegal SapI.rc site found at 9792


Design Notes

Codon optimisation, order of parts in operon, illegal enzyme kinetics.


Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References